The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 12, 2025

Filed:

Feb. 21, 2020
Applicants:

Roche Sequencing Solutions, Inc., Pleasanton, CA (US);

President and Fellows of Harvard College, Cambridge, MA (US);

Inventors:

Aruna Ayer, Santa Clara, CA (US);

George M. Church, Brookline, MA (US);

Mirko Palla, Newton, MA (US);

Francois Pepin, Mountain View, CA (US);

Sukanya Srinivasa Rao Arun Punthambaker, Boston, MA (US);

Peter B. Stranges, Somerville, MA (US);

Assignees:
Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C40B 20/04 (2006.01); C12Q 1/6869 (2018.01); C40B 30/08 (2006.01); C40B 50/06 (2006.01); G01N 33/487 (2006.01); G16B 30/10 (2019.01);
U.S. Cl.
CPC ...
C40B 20/04 (2013.01); C40B 30/08 (2013.01); C40B 50/06 (2013.01); G16B 30/10 (2019.02); C12Q 1/6869 (2013.01); G01N 33/48721 (2013.01);
Abstract

The present disclosure is directed to compositions and methods for deriving a plurality of kinetics parameters () for at least two different enzyme variants in a multiplex manner using nanopore-based sequencing. In some embodiments, the systems and methods may be used to screen different nanopore variants, or different combinations of both nanopore variants and enzyme variants.


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