The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 12, 2025
Filed:
Feb. 17, 2020
University of Macau, Macau, CN;
Yanwei Jia, Macau, CN;
Ren Shen, Macau, CN;
Pui-In Mak, Macau, CN;
Rui Paulo Da Silva Martins, Macau, CN;
University of Macau, Macau, CN;
Abstract
The present disclosure discloses an enhancing agent and a kit for enhancing nucleic acid amplification reaction and a method for performing nucleic acid amplification reaction, relating to the field of biotechnology. The enhancing agent disclosed in the present disclosure has a domain capable of binding to fluorescent dye and a quenching group for quenching fluorescence emitted by the fluorescent dye bound to the domain binds. As the binding affinity between the above domain and the fluorescent dye is lower than the binding affinity between a target amplification product of nucleic acid amplification reaction and the fluorescent dye, the enhancing agent not only can reduce inhibition of high-concentration fluorescent dye to amplification reaction, but also can enhance signal intensity of the fluorescent dye in the nucleic acid amplification reaction based on microfluidic chip, improving reliability of application of the fluorescent dye as amplification indicator on the microfluidic chip.