The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 12, 2025

Filed:

Jul. 14, 2017
Applicant:

The Board of Regents of the Nevada System of Higher Education on Behalf of the University of Nevada, Las Vegas, Las Vegas, NV (US);

Inventors:

Martin R. Schiller, Henderson, NV (US);

Ronald Benjamin, Henderson, NV (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C40B 20/04 (2006.01); C07K 14/005 (2006.01); C12N 15/10 (2006.01); C12N 15/11 (2006.01); C12N 15/113 (2010.01); C12N 15/65 (2006.01); C12N 15/90 (2006.01); C40B 40/08 (2006.01);
U.S. Cl.
CPC ...
C12N 15/907 (2013.01); C07K 14/005 (2013.01); C12N 15/1065 (2013.01); C12N 15/1086 (2013.01); C12N 15/111 (2013.01); C12N 15/113 (2013.01); C12N 15/65 (2013.01); C40B 20/04 (2013.01); C40B 40/08 (2013.01); C12N 2740/16322 (2013.01); C12N 2750/14122 (2013.01);
Abstract

Disclosed are compositions and methods for studying a Tat gene. Specifically, the disclosure provides a vector comprising a double-stranded nucleic acid construct which comprises a Tat gene and a green fluorescent protein (GFP) reporter element, and further wherein the double-stranded nucleic acid construct comprising AAVS1 (adeno-associated virus integration site, a safe harbor locus) arms that flank on both sides of the Tat gene and the reporter element for integration at the human AAVS1 site by homologus recombination. Further provided are methods of using a cell comprising the vector for studying the effects of exogenous conditions on expression of the Tat gene.


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