The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 12, 2025

Filed:

Sep. 08, 2016
Applicants:

Sumitomo Pharma Co., Ltd., Osaka, JP;

Riken, Wako, JP;

Inventors:

Satoshi Ando, Kobe, JP;

Takao Kuroda, Kobe, JP;

Yoshiki Sasai, Kobe, JP;

Assignees:

SUMITOMO PHARMA CO., LTD., Osaka, JP;

RIKEN, Wako, JP;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 5/079 (2010.01); A61K 35/30 (2015.01); C12N 5/10 (2006.01); C12Q 1/02 (2006.01); G01N 33/50 (2006.01); A61K 35/545 (2015.01);
U.S. Cl.
CPC ...
C12N 5/0621 (2013.01); A61K 35/30 (2013.01); C12N 5/10 (2013.01); C12Q 1/02 (2013.01); G01N 33/5014 (2013.01); G01N 33/5058 (2013.01); A61K 35/545 (2013.01); C12N 2500/90 (2013.01); C12N 2501/115 (2013.01); C12N 2501/155 (2013.01); C12N 2501/41 (2013.01); C12N 2501/727 (2013.01); C12N 2506/45 (2013.01); C12N 2513/00 (2013.01);
Abstract

The present invention provides a production method of a retinal cell or retinal tissue, including the following steps: (1) a first step of culturing mammalian pluripotent stem cells in the absence of a feeder cell for a period not exceeding 30 days in a medium comprising 1) a factor for maintaining an undifferentiated state and 2) an MEK inhibitor, (2) a second step of culturing the cells obtained in the first step in suspension to form a cell aggregate, and (3) a third step of culturing the aggregate obtained in the second step in suspension in the presence of a BMP signal transduction pathway activating substance to obtain an aggregate containing retinal cells or a retinal tissue.


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