The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 05, 2025
Filed:
Apr. 20, 2020
Ddbio. Co, Ltd., (Shang Hai), Shanghai, CN;
Chen Zhou, Shanghai, CN;
DDBIO. CO, LTD., (SHANG HAI), Shanghai, CN;
Abstract
Provided is method for producing a phage library displaying antibodies or antibody fragments, comprising providing a first polynucleotide containing LC, a second polynucleotide containing connexon, and a third polynucleotide containing HC, respectively introducing the first, second and third polynucleotides to first, second and third bacteria to obtain a light chain component bacterial library, a connexon component bacterial library, and a heavy chain component bacterial library, obtaining a light chain component plasmid, a connexon component plasmid, and a heavy chain component plasmid from the libraries, obtaining the released LC, released connexon and released HC from the plasmids, connecting the released display vector segments to form a connection product for display, introducing a third bacterium to obtain a display bacterial library, and using the display bacterial library to prepare the phage library for displaying the antibodies or antibody fragments. Also provided is a phage library produced according to the method.