The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 05, 2025

Filed:

Dec. 14, 2018
Applicant:

Cambridge Enterprise Limited, Cambridgeshire, GB;

Inventors:

Chibeza Chintu Agley, Cambridgeshire, GB;

Kevin Chalut, Cambridgeshire, GB;

Jose Carlos Rebelo Da Silva, Cambridgeshire, GB;

Assignee:

Cambridge Enterprise Limited, Cambridgeshire, GB;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C08F 220/56 (2006.01); B82Y 5/00 (2011.01); C08F 222/38 (2006.01); C12M 3/02 (2006.01); C12N 5/00 (2006.01); C12N 5/074 (2010.01); C12N 5/077 (2010.01);
U.S. Cl.
CPC ...
C08F 220/56 (2013.01); C08F 222/385 (2013.01); C12M 3/02 (2013.01); C12N 5/0068 (2013.01); C12N 5/0607 (2013.01); C12N 5/0654 (2013.01); B82Y 5/00 (2013.01); C12N 2501/155 (2013.01); C12N 2506/45 (2013.01); C12N 2533/30 (2013.01);
Abstract

This invention relates to a substrate for mammalian cell culture comprising a co-polymer hydrogel comprising monomeric units of acrylamide, bisacrylamide and a coupling compound, such as 6-acylamidohexanoic acid (6AHA). A cell adhesion molecule, such as a component of the extracellular matrix (ECM), is covalently coupled to the coupling compound monomeric units of the co-polymer. The stiffness and cell adhesion molecule density of the substrate can be independently controlled by altering the concentration of acrylamide and coupling compound in the hydrogel, respectively. Substrates and methods and kits for their production are provided, along with cell culture systems and methods of culturing mammalian cells.


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