Exosome comprising photocleavable protein, and use thereof

Abstract

The present disclosure relates to an exosome comprising a photocleavable protein and a use thereof, and the exosome according to the present disclosure contains a fusion protein comprising a blue fluorescent protein (TagBFP), a photocleavable protein (mMaple3), and an exosome-specific marker protein (CD9), and it has been found that when light of 405 nm is irradiated to the exosome, the photocleavable protein, mMaple3 is cleaved and thereby the blue fluorescent protein in the exosome can be delivered into a target cell. In addition, it has been found that Cre protein in the exosome can be delivered into an animal organ, when light of 405 nm is irradiated to an exosome containing Cre fusion protein (Cre-mMaple3-CD9). Therefore, the exosome containing the photocleavable protein according to the present disclosure is expected to be useful in the protein treatment field by safely and efficiently delivering various therapeutic proteins into cells.