The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 29, 2025
Filed:
Sep. 17, 2021
Euroimmun Medizinische Labordiagnostika Ag, Lübeck, DE;
Stefan Gerlach, Groß Groenau, DE;
Jens Krauth, Lübeck, DE;
Christian Marzahl, Erlangen, DE;
Christopher Krause, Büchen, DE;
Maick Danckwardt, Rondeshagen, DE;
Melanie Hahn, Stockelsdorf, DE;
Jörn Voigt, Lübeck, DE;
EUROIMMUN Medizinische Labordiagnostika AG, Lübeck, DE;
Abstract
There is proposed a method for detecting a binding of autoantibodies from a patient sample to double-stranded deoxyribonucleic acid usingcells by means of fluorescence microscopy, including the steps of: provision of a substrate which has multiplecells, incubation of the substrate with the patient sample which potentially has the autoantibodies, incubation of the substrate with secondary antibodies which have each been labelled with a preferably green fluorescent dye, acquisition of a fluorescence image of the substrate, identification by means of a first pretrained convolutional neural network of respective sub-images in the one fluorescence image that each represent acell, furthermore respective processing of at least one subset of the respective sub-images by means of a second pretrained convolutional neural network for determining respective binding measures which indicate a respective extent of a binding of autoantibodies in a respective kinetoplast region of a respectivecell of a respective sub-image, and determination of an overall binding measure with regard to a binding of autoantibodies from the patient sample to double-stranded deoxyribonucleic acid on the basis of the respective binding measures.