The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 29, 2025
Filed:
Mar. 10, 2021
Alliance for Sustainable Energy, Llc, Golden, CO (US);
Ut-battelle, Llc, Oak Ridge, TN (US);
Gregg Tyler Beckham, Golden, CO (US);
Thelhawadigedara Lahiru Niroshan Jayakody, Wheat Ridge, CO (US);
Adam Michael Guss, Oak Ridge, TN (US);
Thomas David Mand, Oak Ridge, TN (US);
Christopher W. Johnson, Denver, CO (US);
Isabel Pardo Mendoza, Dos Hermanas, ES;
Allison Jean Zimont Werner, Denver, CO (US);
Alliance for Sustainable Energy, LLC, Golden, CO (US);
UT-Battelle, LLC, Oak Ridge, TN (US);
Abstract
Disclosed herein are methods and compositions for catalytic glycolysis to deconstruct PET to bis(2-hydroxyethyl) terephthalate (BHET). For BHET conversion to terephthalate and ethylene glycol, we engineerKT2440 with PETase and MHETase enzymes from. We further engineerto convert terephthalate to a performance-advantaged bioproduct, β-ketoadipic acid, and for improved utilization of ethylene glycol, a byproduct of BHET catabolism. In a bioreactor, we produce 15.1±0.6 g/L of β-ketoadipic acid (βKA) from BHET at 76±3% molar yield. Lastly, we demonstrate conversion of catalytically depolymerized PET to βKA. Overall, this work highlights the potential of tandem catalytic deconstruction and biological conversion as a means to upcycle waste PET.