The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 29, 2025
Filed:
Jul. 31, 2020
Applicant:
Monsanto Technology Llc, St. Louis, MO (US);
Inventor:
Ervin D. Nagy, St. Louis, MO (US);
Assignee:
Monsanto Technology LLC, St. Louis, MO (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 15/52 (2006.01); C12N 15/64 (2006.01); C12Q 1/68 (2018.01); C12Q 1/6811 (2018.01);
U.S. Cl.
CPC ...
C12N 15/52 (2013.01); C12N 15/64 (2013.01); C12Q 1/6811 (2013.01); C07K 2319/00 (2013.01); C12N 2310/20 (2017.05);
Abstract
This disclosure provides compositions and methods for improving site-directed integration of nucleic acids using RNA-guided nucleases coupled with HUH endonucleases. In one aspect, this disclosure provides a ribonucleoprotein comprising: (a) a re-combinant polypeptid comprising: (i) an amino acid sequence encoding a Cas12a nuclease; (ii) an amino acid sequence encoding a linker; and (iii) an amino acid sequence encoding a HUH nuclease; and (b) at least one guide nucleic acid.