The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 22, 2025
Filed:
Feb. 23, 2021
Applicant:
Integrated Dna Technologies, Inc., Coralville, IA (US);
Inventors:
Ashley M. Wood, Ann Arbor, MI (US);
Jordan Rosefigura, Ann Arbor, MI (US);
Vladimir Makarov, Ann Arbor, MI (US);
Assignee:
INTEGRATED DNA TECHNOLOGIES, INC., Coralville, IA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6848 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/686 (2018.01); C12Q 1/6869 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6848 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/686 (2013.01); C12Q 1/6869 (2013.01);
Abstract
The present disclosure provides kits and methods for a targeted NGS library workflow comprising a multiplexed PCR for target amplification followed by an indexing PCR for incorporation of NGS adapter sequences. This workflow enables overlapping primer pairs in a single tube for contiguous coverage over target regions, while simultaneously preventing amplification of both primer dimers and undesirable mini-amplicons that result from overlapping primer pairs.