The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 22, 2025

Filed:

Sep. 19, 2019
Applicant:

Lineage Cell Therapeutics, Inc., Carlsbad, CA (US);

Inventors:

Rekha R. Nair, Mountain View, CA (US);

Stephanie Kayser, Mountain View, CA (US);

Abhirath S. Parikh, Los Angeles, CA (US);

Uzma Shoukat-Mumtaz, Fremont, CA (US);

Erik Michael Whiteley, Concord, CA (US);

Nathan C. Manley, San Jose, CA (US);

Craig R. Halberstadt, Pleasanton, CA (US);

Assignee:

LINEAGE CELL THERAPEUTICS, INC., Carlsbad, CA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 5/079 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0622 (2013.01); C12N 5/0068 (2013.01); C12N 2500/46 (2013.01); C12N 2501/11 (2013.01); C12N 2501/115 (2013.01); C12N 2501/135 (2013.01); C12N 2501/15 (2013.01); C12N 2501/155 (2013.01); C12N 2501/16 (2013.01); C12N 2501/385 (2013.01); C12N 2501/41 (2013.01); C12N 2506/02 (2013.01); C12N 2506/03 (2013.01); C12N 2506/45 (2013.01); C12N 2533/52 (2013.01);
Abstract

Methods for differentiating pluripotent stem cells to neuroectoderm in dynamic suspension culture using small molecule or protein inhibitors of TGFβ/Activin/Nodal signaling and BMP signaling are provided. Also provided are methot and protocols for differentiating pluripotent stem cells such as human embryonic stem cells first to neuroectoderm, then further to glial progenitor cells, and further to oligodendrocyte progenitor cells (OPCs), and compositions obtained thereby. The methods of the present disclosure reproducibly produce neuroectoderm progenitor cells by day 7 of the differentiation process, glial progenitor cells by day 21 of the differentiation process and OPCs by day 42 of the differentiation process.


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