The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 22, 2025

Filed:

Mar. 29, 2019
Applicant:

The Governing Council of the University of Toronto, Toronto, CA;

Inventors:

Aaron Ray Wheeler, Toronto, CA;

Michael Dean Chamberlain, Toronto, CA;

Julian Lucas Lamanna, Toronto, CA;

Michael David Murdoch Dryden, Toronto, CA;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01L 3/00 (2006.01); C12M 1/33 (2006.01); C12M 1/34 (2006.01); C12M 1/42 (2006.01); C12N 1/06 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6881 (2018.01); G01N 1/28 (2006.01); G01N 15/00 (2006.01); G01N 15/10 (2024.01); G01N 15/14 (2006.01); G01N 35/00 (2006.01); B01L 7/00 (2006.01); G01N 1/40 (2006.01); G01N 15/149 (2024.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
B01L 3/502753 (2013.01); B01L 3/502715 (2013.01); C12M 1/33 (2013.01); C12M 1/34 (2013.01); C12M 1/42 (2013.01); C12N 1/066 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6881 (2013.01); G01N 1/28 (2013.01); G01N 15/00 (2013.01); G01N 15/10 (2013.01); G01N 15/1468 (2013.01); G01N 35/00 (2013.01); B01L 3/502761 (2013.01); B01L 3/502792 (2013.01); B01L 7/52 (2013.01); B01L 2200/0647 (2013.01); B01L 2200/0668 (2013.01); B01L 2300/0867 (2013.01); B01L 2300/089 (2013.01); B01L 2400/0421 (2013.01); B01L 2400/0442 (2013.01); C12Q 2565/629 (2013.01); G01N 1/286 (2013.01); G01N 1/40 (2013.01); G01N 2015/1006 (2013.01); G01N 2015/1028 (2024.01); G01N 15/149 (2024.01); G01N 33/5005 (2013.01);
Abstract

A system for identifying and targeting individual cells within a cell population for selective extraction of cellular content. The system includes a digital microfluidic device having at least one hydrophilic site for receiving cells, an imaging system including a stage for receiving the digital microfluidic device and an imaging module for identifying at least one targeted cell among the cells at the at least one hydrophilic site and a pulsed laser source for lysing the targeted cell to produce a lysate. A control system controls the pulsed laser source, the imaging system and the digital microfluidic device and is programmed for coordinating steps of i) moving droplets on the digital microfluidic device, ii) selecting the targeted cell to be lysed located at the hydrophilic site, iii) illuminating the selected targeted cell by the pulsed laser source to lyse the selected targeted cell to produce lysate, and iv) collecting the lysate.


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