The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 15, 2025

Filed:

Mar. 07, 2022
Applicant:

Institute of Medicinal Plant Development (Implad), Cams & Pumc, Beijing, CN;

Inventors:

Jingyuan Song, Beijing, CN;

Lijun Hao, Beijing, CN;

Wenjie Xu, Beijing, CN;

Tianyi Xin, Beijing, CN;

Guihong Qi, Beijing, CN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/82 (2006.01); C12Q 1/6895 (2018.01); G16B 30/00 (2019.01); G16B 35/20 (2019.01); G16B 99/00 (2019.01);
U.S. Cl.
CPC ...
C12N 15/8213 (2013.01); C12Q 1/6895 (2013.01); C12N 2310/20 (2017.05); G16B 30/00 (2019.02); G16B 35/20 (2019.02); G16B 99/00 (2019.02);
Abstract

A method for identifying plant species based on whole genome analysis and genome editing, i.e., a GAGE, includes that screening PAM-carrying sequences from a whole genome of a plant to be identified; aligning the PAM-carrying sequences with genomes of counterfeit species and closely related species, and then selecting sequences that are only present in the genome of the plant to be identified as target sequences; and performing a detection on the target sequences as selected by introducing a genome editing system, wherein based on the target sequences as selected, a CRISPR RNA (crRNA), which acts to guide a Cas protein to bind with a target sequence to form a complex, is designed and synthesized; then the trans-cleavage activity of the Cas protein is activated so that a single strand DNA bearing a fluorescent signal group is cleaved; and the plant species is identified by detecting the fluorescent signal.


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