The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 15, 2025
Filed:
Aug. 25, 2020
Applicant:
Roche Sequencing Solutions, Inc., Pleasanton, CA (US);
Inventor:
Timothy K. Craig, Campbell, CA (US);
Assignee:
Roche Sequencing Solutions, Inc., Pleasanton, CA (US);
Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C07K 14/31 (2006.01); C12N 9/12 (2006.01); C12Q 1/6869 (2018.01); G01N 33/487 (2006.01);
U.S. Cl.
CPC ...
C07K 14/31 (2013.01); C12N 9/1252 (2013.01); C12Q 1/6869 (2013.01); G01N 33/48721 (2013.01);
Abstract
Described herein are variants of alpha-hemolysin having at least one mutation, such as a mutation to a positive charge. In certain examples, the mutation is selected from 0K, A1K, A1R, D2N, S3K, D4K, D4N, K8R, N47K, E70K, S106K, E111N, 127-131G, D128K, K147N, V149K, E287R, M298A, or combinations thereof in the mature, wild-type alpha-hemolysin amino acid sequence. Also provided are compositions including the variants of alpha-hemolysin, nanopore assemblies including the alpha-hemolysin variants, and methods of sequencing nucleic acids incorporating the same.