The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 08, 2025

Filed:

Feb. 20, 2024
Applicant:

Anhui Gsh Bio-tech Co., Ltd., Anhui, CN;

Inventors:

Shanshan Liu, Beijing, CN;

Hui Liu, Beijing, CN;

Wenwen Zhou, Beijing, CN;

Yongfa Qin, Beijing, CN;

Assignee:
Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P 19/40 (2006.01); C12N 9/12 (2006.01); C12N 9/16 (2006.01); C12N 15/70 (2006.01);
U.S. Cl.
CPC ...
C12P 19/40 (2013.01); C12N 9/1229 (2013.01); C12N 9/16 (2013.01); C12N 15/70 (2013.01); C12Y 207/04001 (2013.01); C12Y 207/04003 (2013.01); C12Y 301/03004 (2013.01);
Abstract

The present invention discloses a production method of enzymatic reaction using adenosine instead of ATP. The method comprises the following steps: (1) adding ATP regeneration enzyme, AK enzyme and adenosine in proportion to carry out an enzymatic reaction in an enzymatic reaction system; (2) separating the ATP regeneration enzyme and AK enzyme by either directly separating ATP regeneration enzyme and AK enzyme immobilized in a reaction tank, or separating free ATP regeneration enzyme and AK enzyme by an ultrafiltration membrane in a filter; and (3) separating and purifying the filtrate of step (2) to obtain a product. The disclosed method provides: greatly reduced industrial production costs; faster reaction rate; stable enzyme recovery system that is energy efficient and environmentally friendly; and capability of reusing the byproducts or collecting them for the production of ATP.


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