The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 01, 2025

Filed:

Mar. 24, 2017
Applicant:

President and Fellows of Harvard College, Cambridge, MA (US);

Inventors:

David A. Weitz, Cambridge, MA (US);

Li Sun, Cambridge, MA (US);

John Heyman, Cambridge, MA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/569 (2006.01); B01L 3/00 (2006.01); C12N 5/0781 (2010.01); C12N 5/0783 (2010.01); C12Q 1/6895 (2018.01); G01N 33/573 (2006.01); G01N 33/68 (2006.01);
U.S. Cl.
CPC ...
G01N 33/56972 (2013.01); B01L 3/502792 (2013.01); C12N 5/0635 (2013.01); C12N 5/0636 (2013.01); C12Q 1/6895 (2013.01); G01N 33/573 (2013.01); G01N 33/6863 (2013.01); C12Q 2600/13 (2013.01);
Abstract

The present invention generally relates to fluidic droplets and systems and methods for determining immune or other cells. Some aspects of the invention are generally directed to assays that combine sensitive detection of secreted products with detection of target cell death in droplets containing an effector cell, systems and methods to isolate droplets in which one or more cell interactions have occurred, or systems and methods to generate nucleic acid information from cell interactions. In addition, some embodiments of the invention are generally directed to containing two (or more) cells in droplets, e.g., an effector cell and one or more target cells, and determining various interactions between the cells within the droplets, such as whether the effector cell kills the target cell, whether the effector cell releases antibodies, cytokines or other substances that are able to interact with the target cell or are released in the presence of the target cell, or the like.


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