The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 01, 2025

Filed:

Jul. 13, 2020
Applicant:

Trustees of Tufts College, Boston, MA (US);

Inventors:

Irene Georgakoudi, Winchester, MA (US);

Dimitra Pouli, Medford, MA (US);

Kyle P. Quinn, Fayetteville, AR (US);

Zhiyi Liu, Medford, MA (US);

Assignee:

TRUSTEES OF TUFTS COLLEGE, Boston, MA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
G01N 21/64 (2006.01); C12Q 1/00 (2006.01); G01N 33/58 (2006.01); G06T 7/00 (2017.01); G06T 7/20 (2017.01);
U.S. Cl.
CPC ...
G01N 21/6486 (2013.01); C12Q 1/008 (2013.01); G01N 33/582 (2013.01); G06T 7/0016 (2013.01); G06T 7/20 (2013.01); G01N 2800/7066 (2013.01); G06T 2207/10064 (2013.01); G06T 2207/30024 (2013.01);
Abstract

Methods and corresponding apparatus and systems for assessing cellular metabolic activity are disclosed. In one aspect, a cell can be illuminated with optical radiation in order to cause multi-photon excitation of at least one endogenous metabolic cofactor in that cell and cause the excited metabolic cofactor to emit fluorescent radiation. A detector can be used to detect the fluorescent radiation emitted by the excited endogenous metabolic cofactor. A computer processor can analyze the fluorescent radiation to derive the following parameters: (1) using a computer processor to analyze the intensity of the fluorescent radiation, (2) a fluorescence lifetime of at least one of the excited metabolic cofactor, (3) a parameter indicative of mitochondrial clustering in the cell. These parameters can be used to assess at least one metabolic process of the cell.


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