The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 01, 2025
Filed:
Mar. 03, 2021
Omniome, Inc., San Diego, CA (US);
Corey M. Dambacher, La Jolla, CA (US);
Joseph Rokicki, San Diego, CA (US);
Keunho Ahn, San Diego, CA (US);
Brittany Ann Rohrman, San Diego, CA (US);
Michael Nguyen, San Diego, CA (US);
Kandaswamy Vijayan, San Diego, CA (US);
PACIFIC BIOSCIENCES OF CALIFORNIA, INC., Menlo Park, CA (US);
Abstract
Method and composition for identifying cognate nucleotides in a Sequencing By Binding™ procedure, wherein one or more labeled nucleotides are detected in ternary complexes but never incorporated. Labeled nucleotides can be incorporable nucleotides that contact preformed blocked primed template nucleic acids. Alternatively, labeled nucleotides are labeled non-incorporable nucleotides. Labeled nucleotides, including labeled non-incorporable nucleotides, can be detected in ternary complexes in the same reaction mixture that incorporates a reversible terminator nucleotide to create a blocked primed template nucleic acid. Detection of ternary complexes can take place in the presence of a catalytic metal ion.