The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 01, 2025

Filed:

Jun. 14, 2019
Applicant:

Gen-probe Incorporated, San Diego, CA (US);

Inventors:

Matthias Jost, San Diego, CA (US);

Barbara L. Eaton, San Diego, CA (US);

Ankur Shah, San Diego, CA (US);

Assignee:

GEN-PROBE INCORPORATED, San Diego, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6806 (2018.01); C12N 15/10 (2006.01); C12Q 1/689 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6806 (2013.01); C12N 15/1006 (2013.01); C12Q 1/689 (2013.01);
Abstract

Method of preparing a biological sample appropriate for use in a subsequent in vitro nucleic acid amplification reaction. A biological sample is combined with an alkaline composition that lyses cells and denatures DNA to create a first liquid composition. The first liquid composition is then mixed with a buffer, a detergent, and a solid support that captures DNA to create a second liquid composition. The buffer, detergent, and solid support can be delivered as components of a single reagent. Captured DNA strands can be used as templates in subsequently performed nucleic acid amplification and detection reactions with improved sensitivity.


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