The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 01, 2025

Filed:

Sep. 05, 2024
Applicant:

Beijing University of Chemical Technology, Beijing, CN;

Inventors:

Tianwei Tan, Beijing, CN;

Menglei Li, Beijing, CN;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12P 7/44 (2006.01); C12N 9/02 (2006.01); C12N 9/10 (2006.01); C12N 9/88 (2006.01); C12N 9/90 (2006.01); C12N 15/52 (2006.01); C12N 15/77 (2006.01);
U.S. Cl.
CPC ...
C12P 7/44 (2013.01); C12N 9/0069 (2013.01); C12N 9/1022 (2013.01); C12N 9/1085 (2013.01); C12N 9/88 (2013.01); C12N 9/90 (2013.01); C12N 15/52 (2013.01); C12N 15/77 (2013.01); C12Y 113/11001 (2013.01); C12Y 202/01001 (2013.01); C12Y 401/01061 (2013.01); C12Y 401/01063 (2013.01); C12Y 402/0101 (2013.01); C12Y 402/03004 (2013.01);
Abstract

The present invention discloses a genetic engineering bacterium for de novo synthesis of cis,cis-muconic acid by taking glucose as a substrate and applications thereof, and belongs to the technical field of genetic recombination and metabolic engineering. The genetic engineering bacterium for de novo synthesis of cis,cis-muconic acid (MA) by taking glucose as the substrate disclosed in the present invention is modified with chassis microbes, and includes recombinantfor a cis,cis-muconic acid pathway construction module and an intermediate high-yield module. Production capacity of strains is greatly improved; MA of 90.2 g/L is finally obtained in fermentation liquor; and possibilities are provided for green and low-cost production of numerous chemicals such as adipic acid and nylon-66.


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