The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 24, 2025

Filed:

Aug. 30, 2019
Applicant:

Japan Science and Technology Agency, Saitama, JP;

Inventors:

Itaru Imayoshi, Kyoto, JP;

Mayumi Yamada, Kyoto, JP;

Yusuke Suzuki, Kyoto, JP;

Shinji Nagasaki, Kyoto, JP;

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/62 (2006.01); C07K 14/47 (2006.01); C12N 15/63 (2006.01);
U.S. Cl.
CPC ...
C12N 15/62 (2013.01); C07K 14/4702 (2013.01); C12N 15/63 (2013.01);
Abstract

The present invention provides a photoactivatable Tet-OFF/ON system that can precisely control temporal and spatial gene expression. The present invention is a PA-Tet-OFF/ON system that includes a target gene expression cassette including a TRE having a TetO sequence, a promoter which is controlled by the TRE, and a target gene whose expression is controlled by the promoter; a first fusion protein expression cassette containing a gene which encodes a first fusion protein containing TetR or rTetR and a first protein; and a second fusion protein expression cassette containing a gene which encodes a second fusion protein containing p65AD and a second protein, in which the first protein and the second protein bind to each other to form a heterodimer only in a state of being irradiated with light at a specific wavelength.


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