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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 12331291 B1

PDFFull Text
Date of Patent:
Jun. 17, 2025

Filed:

Jul. 19, 2024

Split complementary base editing systems based on bimolecular deaminases and uses thereof

Applicant:

Sun Yat-sen University, Guangdong, CN;

Inventors:

Jianfeng Li, Guangdong, CN;

Xiangyu Xiong, Guangdong, CN;

Kehui Liu, Guangdong, CN;

Xionglei He, Guangdong, CN;

Assignee:

SUN YAT-SEN UNIVERSITY, Guangzhou, CN;

Attorneys:

Barron & Young PLLC

CHARLES S HO

LYDIA M HO

Primary Examiner:

Anne M Gussow

Assistant Examiner:

Kyle T Rega

Int. Cl.
CPC ...
C12N 15/11 (2006.01); C12N 9/22 (2006.01); C12N 9/78 (2006.01); C12N 15/81 (2006.01); C12N 15/82 (2006.01); C12N 15/85 (2006.01);
U.S. Cl.
CPC ...
C12N 15/111 (2013.01); C12N 9/22 (2013.01); C12N 9/78 (2013.01); C12N 15/81 (2013.01); C12N 15/82 (2013.01); C12N 15/85 (2013.01); C12N 2310/20 (2017.05);
Abstract

Disclosed are split complementary base editing systems based on bimolecular deaminases and uses thereof. A split complementary base editing system mainly includes base editing fusion proteins A and B that are from splitting at a deaminase domain embedded inside a Cas9 nickase (nCas9), and a guide RNA (gRNA). The present disclosures of split complementary cytosine base editing systems can greatly reduce Cas9-dependent and Cas9-independent off-target effects in the genome while maintaining robust on-target cytosine base editing. The present disclosures also provide the split complementary adenine base editing systems. The split complementary cytosine (adenine) base editing systems are widely applicable to various eukaryotic organisms, and can be used in crop genetic breeding, animal breed improvement, and even clinical treatment of human genetic diseases.

Find Patent Forward Citations

Li, Jianfeng. (2025). Split complementary base editing systems based on bimolecular deaminases and uses thereof (U.S. Patent No. 12331291). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/12331291

Data source: USPTO (United States Patent and Trademark Office) public patent records. Weekly synchronization. How IDiyas builds this profile

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