The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 17, 2025

Filed:

Nov. 26, 2024
Applicant:

President and Fellows of Harvard College, Cambridge, MA (US);

Inventors:

David A. Weitz, Cambridge, MA (US);

Jeremy Agresti, Cambridge, MA (US);

Liang-Yin Chu, Cambridge, MA (US);

Jin-Woong Kim, Cambridge, MA (US);

Amy Rowat, Cambridge, MA (US);

Morten Sommer, Cambridge, MA (US);

Gautam Dantas, Cambridge, MA (US);

George M. Church, Cambridge, MA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); C40B 40/06 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1065 (2013.01); C40B 40/06 (2013.01);
Abstract

The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel. For example, a droplet may be hardened to form a gel, where the droplet contains a cell, DNA, or other suitable species. The gel may be exposed to a reactant, and the reactant may interact with the gel and/or with the cell, DNA, etc., in some fashion. For example, the reactant may diffuse through the gel, or the hardened particle may liquefy to form a liquid state, allowing the reactant to interact with the cell. As the DNA is amplified using PCR, some of the DNA will be bound to the gel via the PCR primer.


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