The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 17, 2025

Filed:

Jul. 22, 2022
Applicants:

The Broad Institute, Inc., Cambridge, MA (US);

Massachusetts Institute of Technology, Cambridge, MA (US);

Inventors:

Paul Blainey, Cambridge, MA (US);

Son Hoang, Cambridge, MA (US);

Huaibin (Eli) Zhang, Cambridge, MA (US);

Jillian Nolan, Cambridge, MA (US);

Soohong Kim, Cambridge, MA (US);

Assignees:

THE BROAD INSTITUTE, INC., Cambridge, MA (US);

MASSACHUSETTS INSTITUTE OF TECHNOLOGY, Cambridge, MA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
B01L 3/00 (2006.01); C12N 15/10 (2006.01); C12Q 1/6837 (2018.01); F04B 19/00 (2006.01); F16K 99/00 (2006.01);
U.S. Cl.
CPC ...
B01L 3/502738 (2013.01); B01L 3/5025 (2013.01); B01L 3/502715 (2013.01); B01L 3/502723 (2013.01); B01L 3/50273 (2013.01); C12N 15/1065 (2013.01); C12Q 1/6837 (2013.01); F04B 19/006 (2013.01); F16K 99/0015 (2013.01); F16K 99/0057 (2013.01); F16K 99/0059 (2013.01); B01J 2219/00398 (2013.01); B01J 2219/00495 (2013.01); B01J 2219/00585 (2013.01); B01J 2219/0059 (2013.01); B01J 2219/00689 (2013.01); B01J 2219/00722 (2013.01); B01L 2200/10 (2013.01); B01L 2200/143 (2013.01); B01L 2200/16 (2013.01); B01L 2300/027 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0861 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/0867 (2013.01); B01L 2300/087 (2013.01); B01L 2300/123 (2013.01); B01L 2400/0481 (2013.01); B01L 2400/0655 (2013.01); F16K 2099/0084 (2013.01); F16K 2099/0086 (2013.01);
Abstract

The microfluidic devices and systems disclosed herein reduce sample loss and help decrease sample processing bottlenecks for applications such as next generation sequencing (NGS). The microfluidic devices include a plurality of reaction modules. Each reaction module may comprise one or more reaction circuits. Each reaction circuit may comprise a single reaction flow channel with each reaction circuit connected by a bridge flow channel. Alternatively, each reaction circuit may comprise two or more reaction flow channels connected by two or more bridge flow channels. The combination of any two bridge flow channels and a portion of the two or more reaction flow channels between the any two bridge flow channels defining may define the reaction circuit. The reaction module may be arranged as nodes connected by bridge flow channels or each reaction module may be arranged in a parallel fashion on the microfluidic device.


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