The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 10, 2025

Filed:

Jan. 10, 2023
Applicant:

University of Massachusetts, Boston, MA (US);

Inventors:

Craig Martin, Amherst, MA (US);

Elvan Cavac, Northampton, MA (US);

Kithmie Harshana Malagodapathiranage, Amherst, MA (US);

Shuo Sui, Sunderland, MA (US);

Sarah L. Perry, Belchertown, MA (US);

Yasaman Gholamalipour, Worcester, MA (US);

Assignee:

University of Massachusetts, Boston, MA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12N 9/12 (2006.01); C12N 15/10 (2006.01); C12Q 1/6865 (2018.01);
U.S. Cl.
CPC ...
C12P 19/34 (2013.01); C12N 9/1247 (2013.01); C12N 15/10 (2013.01); C12Q 1/6865 (2013.01);
Abstract

Described herein are synthetic methods for producing sequence-specific RNA oligonucleotides that eliminate impurities produced in prior art methods. In one aspect, an end-protected capture DNA complementary to a portion of the product RNA is employed. In another aspect, the template DNA is covalently or noncovalently linked to the RNA polymerase, either directly or through the use of a nontemplate DNA. In a third aspect, a flow chamber is employed. All of the methods can be used in combination.


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