The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 10, 2025
Filed:
May. 31, 2019
Tsinghua University, Beijing, CN;
Jianbin Wang, Beijing, CN;
Hongwei Wang, Beijing, CN;
Junlong Yin, Beijing, CN;
Meng Tian, Beijing, CN;
Tsinghua University, Beijing, CN;
Abstract
The present invention provides a degradable microbead comprising a polymer molecule crosslinked by a crosslinking agent, wherein the polymer molecule and/or the crosslinking agent comprises a sensitive chemical bond that is cleavable through a chemical and/or light treatment, thereby resulting in the degradation of the degradable microbead. The present invention also provides a method of separating a target protein from a sample. By using the degradation of the degradable microbead to replace an elution step in protein purification, it is possible to select a combination of target protein and affinity ligand with a stronger affinity, thereby improving the protein purification efficiency. The method is especially suitable for the high-throughput preparation of multiple protein samples, for example providing a protein sample for electron microscope observation or mass spectrometry measurement.