The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 27, 2025

Filed:

Mar. 27, 2020
Applicant:

The Hospital for Sick Children, Toronto, CA;

Inventors:

Sheena Bouch, Toronto, CA;

Michael Litvack, Toronto, CA;

Martin Post, Toronto, CA;

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 5/0789 (2010.01); A61K 35/15 (2025.01); C12N 5/071 (2010.01); C12N 5/0786 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0647 (2013.01); C12N 5/0645 (2013.01); C12N 5/0692 (2013.01); A61K 35/15 (2013.01); C12N 2500/02 (2013.01); C12N 2500/90 (2013.01); C12N 2501/125 (2013.01); C12N 2501/155 (2013.01); C12N 2501/165 (2013.01); C12N 2501/22 (2013.01); C12N 2501/2303 (2013.01); C12N 2501/727 (2013.01); C12N 2506/03 (2013.01); C12N 2506/45 (2013.01);
Abstract

A method of differentiating pluripotent stem cells into hemangioblasts comprising incubating the pluripotent stem cells in a first serum-free differentiation medium comprising bone morphogenetic protein 4 (BMP4), vascular endothelial growth factor (VEGF) and stem cell factor (SCF) to induce differentiation of the pluripotent stem cells into hemangioblasts or hemangioblast-containing embryoid bodies is provided. The hemangioblasts or embryoid bodies may be cultured in a second differentiation medium comprising at least granulocyte-macrophage colony stimulating factor (GM-CSF), macrophage colony stimulating factor (M-CSF) and interleukin-3 (IL-3) for a period of time sufficient to generate alveolar-like macrophages.


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