Method and device for identifying specific region in microorganism target fragment and use thereof

Abstract

A method for identifying presence of a microorganism in a sample has two processes. The first process comprises: S, respectively comparing a microorganism target fragment with genome sequences of one or more comparison strain one-to-one, and removing fragments of which the similarity exceeds a preset value, to obtain a plurality of residual fragments as first-round cut fragments T-T; S, respectively comparing the first-round cut fragments T-Twith the remaining comparison strains, and removing fragments of which the similarity exceeds a preset value, to obtain a collection of residual cut fragments as a candidate specific region of the microorganism target fragment; and S, verifying and obtaining a specific region. The second process comprises performing a PCR amplification of the specific region to detect the microorganism. The method is high in accuracy and sensitivity, which can identify the subspecies level and a dual-verification module is provided.