The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 20, 2025
Filed:
Nov. 18, 2019
Depixus, Paris, FR;
Jimmy Ouellet, Lardy, FR;
Jerome Maluenda, Saint Michel S/orge, FR;
DEPIXUS, Paris, FR;
Abstract
The present invention relates to a method of preparing a nucleic acid molecule comprising a target region for isolation from a sample, said method comprising the steps of contacting a population of nucleic acid molecules with a first Class 2 Type V Cas protein-gRNA complex, wherein the gRNA comprises a guide segment that is complementary to a first sequence within a nucleic acid molecule, said first sequence being located adjacent to said target region, thereby forming a Class 2 Type V Cas protein-gRNA-nucleic acid complex, and a protector molecule, contacting the population of nucleic acid molecules with at least one enzyme having exonuclease activity, recovering said nucleic acid molecule comprising the target region from the Class 2 Type V Cas protein-gRNA-nucleic acid complex formed in step a), and contacting the nucleic acid molecule of step c) with a processive polymerase, preferably a mesophilic or thermophilic polymerase, even more preferably a T4 DNA polymerase.