The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 20, 2025

Filed:

May. 17, 2022
Applicant:

Washington University, St. Louis, MO (US);

Inventors:

John DiPersio, St. Louis, MO (US);

Matthew Cooper, St. Louis, MO (US);

Alun Carter, St. Louis, MO (US);

Assignee:

Washington University, St. Louis, MO (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C07K 14/725 (2006.01); A61K 39/00 (2006.01); A61K 40/11 (2025.01); A61K 40/15 (2025.01); A61K 40/22 (2025.01); A61K 40/31 (2025.01); A61K 40/41 (2025.01); A61K 40/42 (2025.01); A61P 35/00 (2006.01); C07K 16/28 (2006.01); C12N 5/0783 (2010.01); C12N 15/90 (2006.01);
U.S. Cl.
CPC ...
C07K 14/7051 (2013.01); A61K 40/11 (2025.01); A61K 40/15 (2025.01); A61K 40/22 (2025.01); A61K 40/31 (2025.01); A61K 40/418 (2025.01); A61K 40/4202 (2025.01); A61K 40/421 (2025.01); A61K 40/4211 (2025.01); A61P 35/00 (2018.01); C07K 16/28 (2013.01); C12N 5/0636 (2013.01); C12N 5/0638 (2013.01); C12N 15/907 (2013.01); A61K 2239/29 (2023.05); A61K 2239/31 (2023.05); A61K 2239/38 (2023.05); A61K 2239/48 (2023.05); C12N 2310/20 (2017.05);
Abstract

Disclosed herein are methods of gene editing, or endogenous suppression, of cytokines/chemokines/transcription factors secreted from chimeric antigen receptor (CAR)-bearing immune effector cell such as CAR-T cells for the mitigation of cytokine release syndrome and/or CAR-T associated neuropathy. These methods involve insertion of the CAR into a locus of a cytokine gene, blocking its expression. Also disclosed herein are (CAR)-bearing immune effector cells with CARs inserted into a locus of a cytokine gene, and methods of treatment of diseases with immunotherapy with a reduced incidence of cytokine release syndrome and/or CAR-T associated neuropathy.


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