The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 13, 2025
Filed:
Dec. 02, 2016
Applicant:
St. Jude Children's Research Hospital, Inc., Memphis, TN (US);
Inventors:
Assignee:
ST. JUDE CHILDREN'S RESEARCH HOSPITAL, INC., Memphis, TN (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C40B 50/06 (2006.01); C07K 14/725 (2006.01); C12N 15/10 (2006.01); C12N 15/66 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1031 (2013.01); C07K 14/7051 (2013.01); C12N 15/10 (2013.01); C12N 15/1003 (2013.01); C12N 15/66 (2013.01); C12N 2310/11 (2013.01); C40B 50/06 (2013.01);
Abstract
The invention provides a method for rapid cloning of T-cell receptors (TCRs) (e.g., paired αβ and γδ TCR chains) and B-cell receptors (BCRs) (e.g. paired IgH or IgK or Igλ) from single cells by CDR3 substitution using single cell PCR products and Gibson Assembly techniques and a pre-generated TCR (or BCR) library in an expression vector.