The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 13, 2025

Filed:

Aug. 21, 2019
Applicant:

University of Florida Research Foundation, Incorporated, Gainesville, FL (US);

Inventors:

Zhonghui Hugh Fan, Gainesville, FL (US);

Kangfu Chen, Gainesville, FL (US);

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
B01D 61/14 (2006.01); B01D 61/18 (2006.01); B01D 63/00 (2006.01); C12M 1/00 (2006.01); C12M 1/12 (2006.01); C12M 1/26 (2006.01); C12M 3/06 (2006.01); G01N 33/543 (2006.01); G01N 33/574 (2006.01);
U.S. Cl.
CPC ...
C12M 33/14 (2013.01); B01D 61/147 (2013.01); B01D 61/18 (2013.01); B01D 63/005 (2013.01); C12M 23/14 (2013.01); C12M 23/16 (2013.01); C12M 25/10 (2013.01); C12M 47/04 (2013.01); G01N 33/54366 (2013.01); G01N 33/57492 (2013.01); B01D 2313/08 (2013.01); B01D 2313/40 (2013.01);
Abstract

A lateral filter array microfluidic (LFAM) device for highly efficient immunoaffinity isolation of target cells from a population of cells. The LFAM device may include of one or more serpentine main channels incorporated with lateral filter arrays. Antibodies are immobilized on the channel surface including the lateral filters and are capable of specific binding to one or more biomolecules on the surface of the target cell. The device may include one or more arrays of lateral filters with different sizes. The overall filters sizes are close to the diameter of the target cell, therefore the interaction between biomarkers on the target cells and corresponding antibodies immobilized on the filter surface is largely strengthened due to the direct contact between target cells and lateral filters. Methods include flowing a population of cells through an antibody-coated LFAM device for target cells capture, followed by washing the device to remove non-specific captured cells.


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