The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 29, 2025
Filed:
Mar. 24, 2023
National Tsing Hua University, Hsinchu, TW;
Chang Chun Plastics Co., Ltd., Taipei, TW;
Chang Chun Petrochemical Co., Ltd., Taipei, TW;
Dairen Chemical Corp., Taipei, TW;
Yu-Chen Hu, Hsinchu, TW;
Nam Ngoc Pham, Hsinchu, TW;
June-Yen Chou, Taipei, TW;
Hsing-Yun Wang, Taipei, TW;
Vincent Jianan Liu, Taipei, TW;
NATIONAL TSING HUA UNIVERSITY, Hsinchu, TW;
Chang Chun Plastics Co., Ltd., Taipei, TW;
Chang Chun Petrochemical Co., LTD., Taipei, TW;
DAIREN CHEMICAL CORP., Taipei, TW;
Abstract
A gene editing system ofincludes a, a first gene editing fragment and a second gene editing fragment. The first gene editing fragment successively includes a first homology arm and a screening gene. The second gene editing fragment is connected to a C-terminus of the first gene editing fragment and includes a second homology arm, a Cas9 expression cassette and a sgRNA cassette. The Cas9 expression cassette successively includes a Cas9 promoter, a Cas9 gene and three nuclear localization sequences. The sgRNA cassette successively includes a sgRNA promoter, a first ribozyme, a targeting sequence, a scaffold and a second ribozyme. The first gene editing fragment and the second gene editing fragment are constructed as a linear fragment for gene editing of a chromosome of the