The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 21, 2025

Filed:

Dec. 27, 2020
Applicant:

Shanghai Ocean University, Shanghai, CN;

Inventors:

Guijun Guan, Shanghai, CN;

Yuyang Chang, Shanghai, CN;

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 33/574 (2005.12); A01K 67/0275 (2023.12); G01N 33/68 (2005.12);
U.S. Cl.
CPC ...
G01N 33/57484 (2012.12); A01K 67/0275 (2012.12); G01N 33/57438 (2012.12); G01N 33/6893 (2012.12); A01K 2217/075 (2012.12); G01N 2333/705 (2012.12); G01N 2400/40 (2012.12);
Abstract

The gene editing technology was used to carry out target knockout of zc3h12b gene of, establishingmissing Zc3h12b protein product. The establishedall show different degrees of liver lesions such as hepatobiliary duct hyperplasia and fusion, hepatocyte steatosis, and fibrosis. With increase of months, significant fatty liver appears with local cyst necrosis, obvious lymphocyte infiltration in liver sinusoids and abnormal increase in number of macrophages. Human tumor markers cytokeratin 19 (CK19), smooth muscle actin (SMA) and glypican-3 (GPC3) positive cells are also detected. It is suggested that ZC3H12B can be used as a treatment target and a biomarker for biliary cystadenoma (BCA), biliary cystadenocarcinoma (BCAC), or fatty liver or liver cancer related to BCA or BCAC. The zc3h12b missingcan be used as an animal model in researches on pathological processes of BCA, BCAC, or fatty liver or liver cancer related to BCA or BCAC.


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