The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 21, 2025

Filed:

Mar. 12, 2019
Applicant:

Regents of the University of Minnesota, Minneapolis, MN (US);

Inventors:

Branden Moriarity, Minneapolis, MN (US);

Beau Webber, Minneapolis, MN (US);

Cara-Lin Lonetree, Minneapolis, MN (US);

Miechaleen Diers, Minneapolis, MN (US);

Mitchell Kluesner, Minneapolis, MN (US);

Walker Lahr, Minneapolis, MN (US);

Emily Joy Pomeroy, Minneapolis, MN (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/078 (2009.12); A61K 39/00 (2005.12); C12N 5/0783 (2009.12); C12N 15/90 (2005.12);
U.S. Cl.
CPC ...
C12N 15/907 (2012.12); A61K 39/4611 (2023.04); A61K 39/4631 (2023.04); A61K 39/464412 (2023.04); C12N 5/0634 (2012.12); C12N 5/0636 (2012.12); C12N 2310/20 (2017.04); C12N 2510/00 (2012.12); C12N 2800/80 (2012.12);
Abstract

Provided herein are methods and systems for targeted gene disruption (knock-out, missense mutation) and targeted gene knock-in in mammalian cells using base editors and guide RNAs (gRNAs) designed to target splice acceptor-splice donor sites. Also provided herein are universally acceptable genetically engineered cells comprising targeted disruptions in immunotherapy-related genes and comprising a CAR/TCR for therapeutic applications.


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