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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 12281301 B1

PDFFull Text
Date of Patent:
Apr. 21, 2025

Filed:

Apr. 25, 2019

Sequencing-based proteomics

Applicants:

The Broad Institute, Inc., Cambridge, MA (US);

Massachusetts Institute of Technology, Cambridge, MA (US);

Inventors:

Feng Zhang, Cambridge, MA (US);

Jonathan Leo Schmid-Burgk, Cambridge, MA (US);

Veit Hornung, Cambridge, MA (US);

Assignees:

THE BROAD INSTITUTE, INC., Cambridge, MA (US);

MASSACHUSETTS INSTITUTE OF TECHNOLOGY, Cambridge, MA (US);

Attorneys:

F. Brent Nix, Esq.

Johnson, Marcou, Isaacs & Nix, LLC

Erin M. Daly, Esq.

Primary Examiner:

Christian C Boesen

Int. Cl.
CPC ...
C40B 30/04 (2005.12); C12N 9/22 (2005.12); C12N 15/10 (2005.12); C12N 15/65 (2005.12);
U.S. Cl.
CPC ...
C12N 15/1065 (2012.12); C12N 9/22 (2012.12); C12N 15/65 (2012.12); C12N 2310/20 (2017.04);
Abstract

The invention provides a cell library for use in detecting protein expression comprising a plurality of cells, wherein each cell comprises a polynucleotide sequence encoding a detectable marker integrated into the genome of the cell in frame with a protein coding gene selected from a set of target genes, wherein the library comprises more than one cell tagged at each target gene, as well as a cell library for use in detecting protein interactions between a protein of interest and a set of target proteins and a cell library for use in detecting protein modifications. The invention also provides methods of constructing a cell library for use in proteomics, as well as methods for sequencing integration sites of a donor sequence inserted into the genome of a cell. Also provided are systems for analysis of proteins in a cell and kits comprising vectors for tagging a population of cells and for performing proteomics studies.

Find Patent Forward Citations

Zhang, Feng. (2025). Sequencing-based proteomics (U.S. Patent No. 12281301). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/12281301

Data source: USPTO (United States Patent and Trademark Office) public patent records. Weekly synchronization. How IDiyas builds this profile

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