The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 14, 2025
Filed:
Oct. 27, 2022
Life Technologies Corporation, Carlsbad, CA (US);
Gordon A. Janaway, Castro Valley, CA (US);
Mark Andersen, Carlsbad, CA (US);
Kornelija Zgonc, Carlsbad, CA (US);
Michael C. Pallas, San Bruno, CA (US);
Marcin Sikora, Burlingame, CA (US);
Casey R. Mcfarland, San Francisco, CA (US);
Ferrier N. Le, San Jose, CA (US);
Haopeng Wang, Arlington, VA (US);
Jian Gong, San Marcos, CA (US);
Gothami Padmabandu, San Diego, CA (US);
Life Technologies Corporation, Carlsbad, CA (US);
Abstract
Methods and systems for quantification of a target nucleic acid in a sample are provided. The method includes forming a plurality of discrete sample portions. Each of the plurality of discrete sample portions comprising a portion of the sample, and a reaction mixture. The method further includes amplifying the plurality of discrete sample portions to form a plurality of discrete processed sample portions. At least one discrete processed sample portion containing nucleic acid amplification reaction products. Fluorescence signals are detected from the at least one of the plurality of discrete processed sample portions to determine a presence of the at least one target nucleic acid. The method also includes determining the respective volumes of the plurality of the plurality of discrete processed sample portions, and estimating the number of copies-per-unit-volume of the at least one target nucleic acid in the sample. Estimating the number of copies-per-unit-volume is based on the number of discrete processed sample portions determined to contain the at least one target nucleic acid therein.