The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 14, 2025

Filed:

May. 01, 2017
Applicant:

Miltenyi Biotec Gmbh, Bergisch Gladbach, DE;

Inventors:

Marion Jurk, Pulheim, DE;

Stefan Wild, Cologne, DE;

Andreas Bosio, Cologne, DE;

Assignee:

MILTENYI BIOTEC B.V. & CO. KG, Bergisch Gladbach, DE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/00 (2005.12); C07K 14/47 (2005.12); C12N 5/07 (2009.12); C12N 5/074 (2009.12); C12N 5/0775 (2009.12); C12N 5/0783 (2009.12); C12N 5/0789 (2009.12); C12N 5/10 (2005.12); C12N 15/87 (2005.12);
U.S. Cl.
CPC ...
C12N 15/87 (2012.12); C07K 14/4703 (2012.12); C12N 5/0636 (2012.12); C12N 5/0662 (2012.12); C12N 5/0696 (2012.12); C12N 5/10 (2012.12);
Abstract

The present invention provides the use of a nucleic acid encoding SOCS1 for enhancing the efficacy of introducing at least one nucleic acid of interest into a cell; a method of repeated transfection of a cell with at least one nucleic acid of interest comprising the steps of adding a) nucleic acid encoding SOCS1, and simultaneously or subsequently b) at least one nucleic acid of interest encoding at least one polypeptide of interest, wherein at least step b) is repeated at least once; and a method of electroporation of a cell with at least one nucleic acid of interest comprising the steps of adding to the cell a) a nucleic acid encoding SOCS1, and simultaneously or subsequently b) said at least one nucleic of interest. The at least one nucleic acid of interest and the nucleic acid encoding SOCS1 may be mRNAs, wherein each of said mRNAs has a poly(A) tail at its 3' end comprising at least 200 adenines.


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