The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 14, 2025
Filed:
Jan. 06, 2020
Jiangnan University, Wuxi, CN;
Shandong Runde Biotechnology Co., Ltd., Taian, CN;
Long Liu, Wuxi, CN;
Jian Chen, Wuxi, CN;
Xueqin Lv, Wuxi, CN;
Jianxing Lu, Taian, CN;
Changfeng Liu, Taian, CN;
Guocheng Du, Wuxi, CN;
Jianghua Li, Wuxi, CN;
Chen Deng, Taian, CN;
Jiangong Lu, Taian, CN;
JIANGNAN UNIVERSITY, Wuxi, CN;
SHANDONG RUNDE BIOTECHNOLOGY CO., LTD., Taian, CN;
Abstract
The present invention discloses a RamA transcription factor mutant for promoting the production of N-acetylglucosamine and use thereof. The mutant is obtained by mutating lysine at position 90 to asparagine and serine at position 92 to lysine in a parent having an amino acid sequence as shown in SEQ ID NO: 2. The present invention provides a genetically engineered strain that overexpresses the RamA transcription factor mutant and increases the production of N-acetylglucosamine. By overexpressing the transcription factor RamA that is involved in the regulation of carbon metabolism, the extracellular accumulation of N-acetylglucosamine is increased, with a maximum concentration reaching 31.5 g/L, which lays a foundation for further metabolic engineering ofto produce glucosamine. The method for constructing recombinantof the invention is simple, and convenient in use, and thus has good application prospects.