The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 08, 2025

Filed:

Apr. 01, 2020
Applicant:

Universität Für Bodenkultur Wien, Vienna, AT;

Inventors:

Domen Zavec, Vienna, AT;

Brigitte Gasser, Vienna, AT;

Diethard Mattanovich, Vienna, AT;

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/81 (2006.01); C07K 14/765 (2006.01); C07K 16/06 (2006.01); C12N 9/04 (2006.01);
U.S. Cl.
CPC ...
C12N 15/815 (2013.01); C07K 14/765 (2013.01); C07K 16/06 (2013.01); C12N 9/0006 (2013.01); C12Y 101/01001 (2013.01); C12Y 101/03013 (2013.01); C07K 2317/14 (2013.01); C07K 2317/569 (2013.01); C12N 2510/02 (2013.01); C12N 2830/002 (2013.01);
Abstract

A recombinant methanol utilization pathway deficient methylotrophic yeast (Mut−) host cell which is engineered: a) by one or more genetic modifications to reduce expression of a first and a second endogenous gene compared to the host cell prior to said one or more genetic modifications, wherein i. the first endogenous gene encodes alcohol oxidase 1 (AOX1) comprising the amino acid sequence identified as SEQ ID NO:1 or a homologue thereof, and ii. the second endogenous gene encodes alcohol oxidase 2 (AOX2) comprising the amino acid sequence identified as SEQ ID NO:3 or a homologue thereof, and b) by one or more genetic modifications to increase expression of an alcohol dehydrogenase (ADH2) gene compared to the host cell prior to said one or more genetic modifications, wherein the ADH2 gene encodes an alcohol dehydrogenase (ADH2).


Find Patent Forward Citations

Loading…