The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 08, 2025

Filed:

Dec. 27, 2019
Applicant:

Wilmar (Shanghai) Biotechnology Research & Development Center Co., Ltd, Shanghai, CN;

Inventors:

Wei Wu, Shanghai, CN;

Xiaojun Dai, Shanghai, CN;

Haisheng Cao, Shanghai, CN;

Meifeng Zhou, Jiaxing, CN;

Qiwen Niu, Shanghai, CN;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 1/16 (2006.01); C12N 9/16 (2006.01); C12N 15/81 (2006.01); C12P 7/649 (2022.01); C12R 1/84 (2006.01);
U.S. Cl.
CPC ...
C12N 1/165 (2021.05); C12N 9/16 (2013.01); C12N 15/815 (2013.01); C12P 7/649 (2013.01); C12R 2001/84 (2021.05);
Abstract

Provided is amutant strain for expressing an exogenous gene. Specifically, provided is amutant strain comprising, with respect tomutant strain GS115 or CICC32806, one or more of the following six mutations: BQ9382_C1-2260, EKK deletions at positions 308-310, a hypothetical protein; BQ9382_C1-3800, E129K, 60S ribosomal subunit assembly/exported protein LOC1; BQ9382_C1-5700, 1312M, mitochondrial external NADH dehydrogenase, type II NAD(P)H: quinone oxidoreductase; BQ9382_C2-3950, Q145X, an essential protein having a binding partner Psr1p and used for completely activating a general stress response; BQ9382_C3-2220, E188K, a hypothetical protein; and BQ9382_C3-4370, W196X, orotidine 5\'-phosphate decarboxylase. The providedmutant strain is an effective commonly employed host for exogenous expression, and can efficiently express different proteins, especially phospholipase and lipase.


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