The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 01, 2025

Filed:

Jan. 29, 2021
Applicants:

The Broad Institute, Inc., Cambridge, MA (US);

Massachusetts Institute of Technology, Cambridge, MA (US);

President and Fellows of Harvard College, Cambridge, MA (US);

Inventors:

Xin Jin, Cambridge, MA (US);

Paola Arlotta, Cambridge, MA (US);

Aviv Regev, Cambridge, MA (US);

Feng Zhang, Cambridge, MA (US);

Sean Simmons, Cambridge, MA (US);

Assignees:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C07H 21/02 (2006.01); C12N 15/90 (2006.01); C12Q 1/6883 (2018.01); C12Q 1/6897 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6883 (2013.01); C12N 15/907 (2013.01); C12Q 1/6897 (2013.01); C12N 2310/20 (2017.05); C12N 2800/80 (2013.01); C12Q 2600/136 (2013.01);
Abstract

Described herein are methods and uses thereof for in vivo evaluating functions of multiple genes in parallel by combining in utero genetic perturbation of progenitor cells and single-cell transcriptomic profiling of progeny cells in animals. These methods can be used, among other things, to reveal in vivo gene functions in a cell type-specific manner.


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