The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 01, 2025
Filed:
Aug. 15, 2019
Roche Sequencing Solutions, Inc., Pleasanton, CA (US);
Kapa Biosystems, Inc., Wilmington, MA (US);
Daniel Burgess, Black Earth, WI (US);
Brian Christopher Godwin, Livermore, CA (US);
Alexander Lovejoy, Newark, CA (US);
Bronwen Miller, Cape Town, ZA;
Jo-Anne Elizabeth Penkler, Cape Town, ZA;
Joseph Platzer, Oakland, CA (US);
Martin Ranik, Santa Clara, CA (US);
Roche Sequencing Solutions, Inc., Pleasanton, CA (US);
Kapa Biosystems, Inc., Wilmington, MA (US);
Abstract
The present disclosure provides a method for enrichment of at least one target nucleic acid in a library of nucleic acids. A first oligonucleotide is hybridized to a target nucleic acid in library of nucleic acids having first and second adapters. The hybridized first oligonucleotide is extended with a first polymerase, thereby producing a first primer extension complex including the target nucleic acid and the extended first oligonucleotide. The first primer extension complex is captured, enriched relative to the library of nucleic acids, and a second oligonucleotide is hybridized to the target nucleic acid. The hybridized second oligonucleotide is extended with a second polymerase, thereby producing a second primer extension complex including the target nucleic acid and the extended second oligonucleotide, and further liberating the extended first oligonucleotide from the first primer extension complex.