The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 01, 2025
Filed:
Jan. 17, 2020
Sekisui Chemical Co., Ltd., Osaka, JP;
Sekisui Medical Co., Ltd., Tokyo, JP;
Takamasa Kouno, Osaka, JP;
Nobuhiko Inui, Saitama, JP;
Tsutomu Nakamura, Osaka, JP;
Tomoya Sasaki, Saitama, JP;
Sou Yamaguchi, Tokyo, JP;
Katsura Uchida, Tokyo, JP;
SEKISUI CHEMICAL CO., LTD., Osaka, JP;
SEKISUI MEDICAL CO., LTD., Tokyo, JP;
Abstract
Provided is a nucleic acid isolation method whereby it becomes possible to extract a nucleic acid in a simple manner without the need to use an alcohol. A nucleic acid isolation method, comprising the steps of: mixing a specimen containing a nucleic acid with an extraction solution to disperse the nucleic acid in the extraction solution; bringing the extraction solution containing the nucleic acid into contact with an anionic adsorbent; bringing a washing solution into contact with the anionic adsorbent; and bringing a collection solution into contact with the anionic adsorbent, the extraction solution containing a protein denaturant, the washing solution containing a basic compound and having a pH value equal to or less than a pKa value of a conjugate acid of the basic compound, and the collection solution having a pH value equal to or more than the pKa value of the conjugate acid of the basic compound.