Systems, methods and computer programs for a microscope system and for determining a transformation function

Abstract

Examples relate to systems, methods and computer programs for a microscope system and for determining a transformation function, and to a corresponding microscope system. The system for the microscope system comprises one or more processors and one or more storage devices. The system is configured to obtain first imaging sensor data from a first imaging sensor of a microscope of the microscope system and second imaging sensor data from a second imaging sensor of the microscope, the first imaging sensor data comprises sensor data on light sensed in a first plurality of mutually separated wavelength bands. The second imaging sensor data comprises sensor data on light sensed in a second plurality of mutually separated wavelength bands. The wavelength bands of the first plurality of mutually separated wavelength bands or of the second plurality of mutually separated wavelength bands are wavelength bands that are used for fluorescence imaging. The system is configured to generate a composite color image based on the first imaging sensor data and based on the second imaging sensor data. The composite color image is based on a plurality of color channels. The composite color image is generated using a transformation function to define a transformation to be performed between the imaging sensor data and the composite color image, such that the composite color image is generated using sensor data on light sensed in each wavelength band of the first and second plurality of mutually separated wavelength bands.