The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 25, 2025

Filed:

Dec. 07, 2021
Applicant:

Bio-rad Laboratories, Inc., Hercules, CA (US);

Inventors:

Kenneth Oh, Pleasant Hill, CA (US);

Carl Marlowe, San Francisco, CA (US);

Stephen L. Swihart, Walnut Creek, CA (US);

Evan Thrush, San Anselmo, CA (US);

Michael Griffin, El Cerrito, CA (US);

Assignee:

Bio-Rad Laboratories, Inc., Hercules, CA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 27/447 (2006.01); G01N 21/64 (2006.01); G01N 33/58 (2006.01); G01N 33/68 (2006.01);
U.S. Cl.
CPC ...
G01N 27/44726 (2013.01); G01N 21/6456 (2013.01); G01N 33/6827 (2013.01); G01N 2021/6439 (2013.01);
Abstract

Methods and reference compositions for quantifying protein using tagged standards. In an exemplary method, a reference composition and a protein may be electrophoresed in respective lanes of a gel. The reference composition may include quantitation standards of different size and each including a tag present at a different concentration. The quantitation standards and the protein may be transferred from the gel to a solid support to create a blot. Luminescence may be detected from the blot to obtain respective luminescence values separately representing an abundance of the tag of each quantitation standard and an abundance of the protein. A quantity of the protein may be determined using the respective luminescence values.


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