The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 25, 2025

Filed:

Nov. 14, 2019
Applicant:

Cairn Biosciences, Inc., San Francisco, CA (US);

Inventors:

Mary J. C. Ludlam, San Francisco, CA (US);

David Wartmann, Berkeley, CA (US);

Ciara Gallagher, Redwood City, CA (US);

Assignee:

Cairn Biosciences, Inc., San Francisco, CA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12M 1/34 (2006.01); C12M 1/00 (2006.01); C12M 1/42 (2006.01); C12M 3/06 (2006.01); C12N 5/09 (2010.01); G01N 21/64 (2006.01);
U.S. Cl.
CPC ...
C12M 41/36 (2013.01); C12M 23/16 (2013.01); C12M 23/20 (2013.01); C12M 29/00 (2013.01); C12M 35/04 (2013.01); C12M 35/08 (2013.01); C12M 41/46 (2013.01); C12M 47/04 (2013.01); C12N 5/0693 (2013.01); G01N 21/6428 (2013.01); G01N 21/6452 (2013.01); C12N 2500/00 (2013.01); C12N 2527/00 (2013.01); G01N 2021/6439 (2013.01);
Abstract

A method for dynamic evolution and/or adaptation and monitoring of characteristics in living cells is provided, wherein the method may be performed at a microfluidic-enabled cell-culture device comprising pneumatic layer for directing flow of fluid to a plurality of individually addressable wells, and one or more sensors configured to detect data regarding environments inside one or more of the plurality of wells. The method may involve culturing a population of cells in a first well of the plurality of wells, perturbing one or more characteristics of an environment in the first well following the culturing of the population of cells, monitoring one or more characteristics of the population of cells in the first well, and removing all or part of the evolved/adapted population of cells from the first well.


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