The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 25, 2025

Filed:

Sep. 27, 2019
Applicant:

Centrillion Technology Holdings Corporation, Grand Cayman, KY;

Inventors:

Moti Jain, Palo Alto, CA (US);

Wei Zhou, Palo Alto, CA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07H 19/10 (2006.01); C07H 19/20 (2006.01); C07H 21/04 (2006.01); C12Q 1/6869 (2018.01); C07H 19/14 (2006.01); C07H 19/173 (2006.01);
U.S. Cl.
CPC ...
C07H 19/10 (2013.01); C07H 21/04 (2013.01); C12Q 1/6869 (2013.01); C07H 19/14 (2013.01); C07H 19/173 (2013.01); C07H 19/20 (2013.01);
Abstract

The present disclosure provides methods of sequencing polynucleotides and compounds, compositions useful for sequencing of polynucleotides. The chemical compounds include nucleotides and their analogs which possess a sugar moiety comprising a cleavable chemical group capping the 3′-OH group and a base that is attached to a detectable label through a cleavable linker comprising a disulfide bond. In addition, the disulfide bond(s) can be cleavable by a reducing reagent. In addition, after the disulfide bond(s) is/are cleaved by the reducing reagent, there is no free thiol group linked to the nucleotides. Examples of chemical compounds according to the present disclosure are shown as Formulae (IV) and (V).


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