The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 25, 2025
Filed:
Mar. 13, 2024
China University of Mining and Technology, Jiangsu, CN;
China University of Mining and Technology, Xuzhou, CN;
Abstract
A method for purifying biological dust suppressant by two-stage ultrafiltration is provided, which utilizes the differences of physical characteristics such as solubility and molecular morphology of each substance in fermentation broth to separate and purify the target product by two-stage ultrafiltration. Firstly, the fermentation broth is centrifugally pretreated to separate insoluble substances such as microbial cells; after centrifugation, the supernatant passes through a primary ultrafiltration membrane, and the polysaccharide, lactic acid and aggregated biological dust suppressant molecules with molecular weights more than 20 kilodaltons are cut off; organic solvents such as methanol and ethyl acetate are added to the retentate for dilution, further filtering is carried out with ultrafiltration membrane with a cutoff molecular weight more than 10 kilodaltons to obtain liquid product, and finally preparing solid purified product is conducted through rotary evaporation and constant temperature drying.